Redefining Molecular Evolution
Primrose Bio’s Function Generator™ technology boosts microbe and protein performance through genome diversification, improving hosts for producing desired biologics. This ultra-high throughput method accelerates genetic development to optimize manufacturing efficiency and sustainability.
How It Works
Function Generator fuses thousands of protein-coding sequences from microbial genomes in random combinations to generate tens of millions of previously unobtainable novel genes. The formation of new protein architectures are relatively rare in nature and our technology bypasses this evolutionary bottleneck resulting in orders of magnitude leaps in activity improvements.
Once these genes are assembled in a Function Generator library, they are reintroduced into the original host with each transformed cell receiving one new gene combination, and then screened to isolate a desirable trait. Case studies and programs for improved protein synthesis and solubility, resistance or tolerance to toxic products, and/or enhanced productivity and metabolic efficiency have been performed.
novel genes and functions
We improve the function of enzymes and active proteins, and the organisms used to produce them, to achieve robust, economic, and sustainable manufacturing. Our platform delivers dramatic increases in key performance indicators through a novel, whole genome-based protein engineering methodology.
Microbe
Microbial Genome
Protein-coding sequences are mined from a genome
Precisely Generated Input Sequences
Thousands of input sequences are generated in vitro
Collection of Novel Genes
Input sequences are randomly combined to create tens of millions of novel genes
New Sequences
The library of novel genes is introduced back into the microbe
Diversified Organisms with Traits of Interest
Novel and Improved Proteins
Capabilities
Traditionally, optimizing microbes and enzymes is slow and expensive. We remove multiple bottlenecks in development to facilitate rapid and cost-effective genome improvement.
An entire genome’s worth of coding sequences is used to create novel genes and functions to accelerate the development of more productive organisms by delivering higher gains in the synthetic biology design-build-test-learn cycle.
How is this useful?
Broadly applicable, Function Generator can drive productivity and performance improvements in species, including prokaryotes, phage and mammalian cells, that are used across multiple industries.
Applications
Applications have been demonstrated in therapeutic proteins, enzymes, fuels and chemicals food and feed ingredients and fermentation products.
Successes
Our Function Generator platform achieves notable gains in therapeutic protein production and pharmaceutical manufacturing, with successes including enhanced protein solubility, better utilization of modified substrates, and improved RNA polymerase catalytic activity.
Stress & Product Tolerance
Trait or property
Improved Protein Expression
Trait or property
Enhanced protein solubility
‘Recalcitrant’ protein production
E. coli
High (no detectable expression in wild type)
Therapeutic protein production
Therapeutic protein production
Production Strains
Trait or property
Enzyme Improvement
Trait or property
Catalytic activity
Partner Proprietary
Improvements in RNA polymerase performance
mRNA manufacturing