Recombinant CRM197 made in Pfenex Expression Technology®
Research and GMP grade, from milligrams to kilograms.
Research grade
Add to cart, or request a quote for larger quantities.
New customer? Get a free CRM197 sample with code newcustomer at checkout. Shipping not included.
Lyophilized 1 mg
$250.00Add to cart
Lyophilized 20 mg
$2,000.00Add to cart
Frozen 20 mg
$2,000.00Add to cart
| Description | PeliCRM197® diphtheria toxin mutant, recombinant. A non-toxic mutant with a single amino acid substitution of glycine to glutamic acid at position 52.2 Research grade. |
|---|---|
| Source | Pseudomonas fluorescens |
| Purity | > 95% CRM197 by RP-HPLC |
| Endotoxin | < 50 EU/mg of protein |
| Dimers and HMW impurities | < 5% by SE-HPLC |
| Lyophilized | Store at 2 to 8 °C. Reconstitute with sterile deionized water before use. |
| Frozen | Store below -60 °C. Thaw before use. |
Need GMP-grade CRM197?GMP-Grade PeliCRM197® is available now.
Frequently Asked Questions
What is CRM197?
A non-toxic mutant of diphtheria toxin. One amino acid change, glycine to glutamic acid at position 52, removes its toxicity. It is used as a carrier protein in conjugate vaccines.
How is PeliCRM197® made?
It is recombinant CRM197 produced in Pseudomonas fluorescens using Pfenex Expression Technology®.
Research grade or GMP grade?
Research grade ships lyophilized (1 mg or 20 mg) or frozen (20 mg). GMP grade is produced under applicable GMP guidelines as an active substance starting material, supported by a Drug Master File with the US FDA.
What purity does research grade meet?
Greater than 95% CRM197 by RP-HPLC, less than 5% dimers and high-molecular-weight impurities by SE-HPLC, and endotoxin below 50 EU/mg.
Is Pfenex-produced CRM197 in approved vaccines?
Yes. CRM197 produced in the Pfenex platform is in CAPVAXIVE®, VAXNEUVANCE®, Pneumosil® and MenFive®.
What quantities are available?
Milligrams to kilograms. Order research grade online, or request a quote for larger amounts.
References
- 1.Analytical Comparability Assessments of 5 Recombinant CRM197 Proteins from Different Manufacturers and Expression Systems. Hickey, J.M., et al. Journal of Pharmaceutical Sciences, July 2018.
- 2.The amino-acid sequence of two non-toxic mutants of diphtheria toxin: CRM45 and CRM197. Giannini, G., et al. Nucleic Acids Research, May 25, 1984.
CAPVAXIVE® and VAXNEUVANCE® are registered trademarks of Merck. Pneumosil® and MenFive® are registered trademarks of Serum Institute of India Pvt. Ltd.
