High-yield microbial expression, engineered
for scalable biologic production
A proven expression platform with a 20-year track record across
approved therapeutics, vaccines, and global tech transfers.
Pfenex Expression Technology® is a Pseudomonas fluorescens–based platform designed to move efficiently from
construct to production strain and transferable manufacturing process.
WHY PFENEX
CORE ADVANTAGES
Robust, High-Yield Expression
- High titers across a variety of recombinant proteins
- Exceptional performance with antibody fragments and engineered proteins.
- High success rate producing correctly folded, soluble lead proteins
Development Speed
- High-throughput screening and structured workflows
- Rapid progression from construct to production strain
- Integrated analytics supporting faster go/no-go decisions
Scalable, Validated Process
- Designed for seamless scale-up and tech transfer
- Compatible with standard microbial infrastructure
- Proven across clinical and commercial programs
HOW the PFENEX platform WORKS
Strain Engineering, Screening & Expression Strategy
- High-throughput screening of gene–vector–strain combinations
- Vector, promoter, and expression optimization
- Cytoplasmic, periplasmic, and extracellular localization evaluation
- 2 L fermentation scouting
Once a lead strain is identified, we establish a Research Cell Bank (RCB) to support reproducible development.
Protein Secretion
Direct recovery from the culture medium simplifies
purification and improves product quality.
Non-Canonical Amino Acid (ncAA) Incorporation
High-titer production of ncAA containing molecules.
Research Cell Bank Establishment
A stable, reproducible production strain ready for pre-clinical manufacturing and to support GMP cell bank production.
- Cell bank generation
- Identity, purity, viability, and identity confirmation
- Stability characterization
Process Development
Build a manufacturing process that survives tech transfer, provides sufficient material for all phases of development and holds at commercial scale.
Upstream Development
- Internal fermentation capacity from 1L to 100L working volume
- Process parameters tuned for maximum production, not just expression
- Inherent P. fluorescens robustness enables production up to 180,000L
Downstream Processing & Purification
- Recovery and clarification tuned to your product’s critical quality needs
- Chromatography designed for purity at commercial throughput
- Periplasmic expression results in cleaner starting material
Integrated Analytical Methods
- Methods developed simultaneously with the process, not after
- Product quality characterization supports regulatory requirements
- Methods travel with the process during tech transfer
Technology Transfer
Your fully documented process is transferable to any capable CDMO or internal manufacturing site.
- Complete process documentation provided
- Hands on, person-in-plant transfer support
- Backed by 25+ global tech transfers, including major pharmas