ABSTRACT:
Efficient production of vaccine carrier proteins and antigens is paramount to ensuring access to affordable conjugate vaccines. Streamlined conjugation technologies improve product quality and consistency and allow lower antigen input needs. Development of extracellular (EC) localization and non-canonical amino acid (ncAA) incorporation within Primrose Bio’s Pseudomonas fluorescens-based expression platform accelerates the discovery and development of affordable vaccine candidates.
We have developed engineered host strains for EC localization that move properly folded proteins from the periplasmic space to the culture medium where they can be readily separated from microbial cell contaminants without a cell lysis step, reducing downstream purification steps.
Contrary to what has been observed with other recombinant protein expression platforms, P. fluorescens has been shown to be a very robust protein expression engine, capable of incorporating ncAAs with high fidelity and high titers. In some examples, we have demonstrated titers of up to 15 g/L target protein with precise, 100% ncAA incorporation.